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Study of Relationship between Enterococci and Antibiotic Resistance Patterns Among the Clinical Isolates of Enterococcus faecalis.
The resistance patterns among clinical isolates of Enterococcus faecalis are not known due to the lack of isolation and identification of this species from India. The present study aimed to isolate and identify the enterococci, characterize their antimicrobial susceptibility patterns, and correlate the susceptible isolate as a precautionary measure. A total of 102 clinical isolates were obtained in the Microbiology Department of Parshuram Rajan Dental College and Hospital (PRDCH), Pune, India from June 2011 to June 2012. The isolates were identified using Gram stain, colony morphology, Gram staining, biochemical reactions, catalase test, and microscopic morphology. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion method. A standard protocol was followed for characterization of isolates. The isolates were identified as E. faecalis, and all the isolates were identified as Enterococcus species. Resistance was observed against ampicillin, clindamycin, and vancomycin. Resistance was seen in 13.68% of the isolates toward teicoplanin and tetracycline. Out of 102 isolates, 32 (31.35%) were found to be resistant to at least one antibiotic. Resistance was higher in tetracycline (14.63%), followed by vancomycin (8.82%) and ampicillin (7.84%). Most of the strains were resistant to clindamycin (34.52%). The resistance to clindamycin was significantly correlated with the
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