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Haemophilus influenzae is a common cause of human bacterial pneumonia and conjunctivitis. Restriction fragment length polymorphism (RFLP) analysis of plasmid and chromosomal DNA is well suited to the study of this organism. However, it is time-consuming and costly to obtain sufficient quantity of DNA for such analysis. We describe here a procedure using the restriction enzyme Pst I to prepare DNA cleavage patterns by electrophoresis on pulse-field gels. This simplifies the analysis and may reduce the cost of analyses. The method is particularly suitable to the rapid and efficient analysis of large numbers of isolates.Location
Features
Room type
Entire home
Property Type
House
Accommodates
8
Bedrooms
Beautiful, spacious, private house with a 5-acre, wooded property.
The home has plenty of space for the family to hang out and enjoy each other’s company. Big deck out back with a grill and fire pit, and inside space to lounge about. Features a stone fireplace in the living room, as well as a chic, contemporary dining room.
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