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Record of Grancrest War Season 1 (?) english · Full cast and crew on IMDb. Wes and Maddie share a wish: to have a normal family life.
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Sequential, multistep phosphorylation cycles of nerve growth factor (NGF) receptor as revealed by rapid endogenous NGF-stimulated phosphorylation of p135 in PC12 cells.
A fully active NGF receptor, with high NGF binding affinity and guanine nucleotide-stimulated transducibility, has been produced in transfected PC12 cells (Kaufman, L. R., Lappalainen, A. L., Downward, J. D., Lilley, D. F., Adelman, S. P., and Ramer, M. G. (1985) Nature 316, 695-699). The effects of NGF on endogenous NGF receptor phosphorylation were investigated in this study. When PC12 cells were incubated with 2 nM NGF, the NGF receptor was rapidly phosphorylated. Within 5 min, it phosphorylated three serine residues (Ser32, Ser46, and Ser80) of the p135 subunit. Later, within 15 min, the receptor phosphorylated four additional serine residues (Ser75, Ser76, Ser77, and Ser79) of the p75 subunit. The receptor displayed both rapid and late phosphorylation events, as well as autophosphorylation. The addition of 0.1 microM pertussis toxin blocked most of the NGF-stimulated phosphorylation of both the p75 and the p135 subunit. But the addition of a truncated form of the pertussis toxin lacking the COOH-terminal alpha subunit, either alone or together with pertussis toxin, selectively blocked the NGF-
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