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We have cloned a novel transmembrane protein, pre-B cell differentiation antigen 1 (pre-BCD1), which is expressed on B cells before the initiation of B cell differentiation. Pre-BCD1 is expressed on all B cell precursors, on many peripheral B cells, on splenic marginal zone B cells, but not on follicular mantle B cells. By immunohistochemistry, the expression of pre-BCD1 on B cells is restricted to the surface membrane and cytoplasm of B cells. The restricted expression of pre-BCD1 correlates with the presence of the CD21 molecule in the B cells. Analysis of B cell development in the spleen of pre-BCD1(-)/- mice demonstrated that the pre-BCD1(-)/- splenic B cells express the differentiation antigen, B220, but lack other typical markers for immature or mature B cells, such as B220, CD43, CD22, and IgD. In contrast to pre-BCD1(+/+), pre-BCD1(+/-) and pre-BCD1(-)/- splenic B cells, which are all viable and functionally able to differentiate into
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