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[The effect of astragalus membranaceus on acute lung injury caused by intestinal ischemia-reperfusion in rats]. To study the effect of Astragalus membranaceus (AM) on the acute lung injury (ALI) induced by intestinal ischemia-reperfusion (I/R) in rats. Thirty SD rats were randomly divided into 5 equal groups: (1) Sham group; (2) Sham + AM group; (3) I/R group; (4) I/R + AM group; (5) AM group. (1) Lung function: After the rats were anaesthetized, the ligature of the celiac artery and superior mesenteric artery were surgically performed. At the end of 90 min reperfusion, the blood flow of the right femoral artery was interrupted for 1 min, and the reperfusion was performed for 1 h. Then the blood flow of the right femoral artery was reperfused for 1 h again. The lung function was measured at the end of the ischemia, 30 min and 60 min of reperfusion. The lung tissue was stained with HE. (2) Bronchoalveolar lavage: The blood in the trachea was first cleaned off by aspiration, and then the trachea was ligated at one end and opened at the other end. The air in the lung was sucked out by aspiration before the trachea was ligated. Then the lung was perfused with 20 mL of normal saline in the right ventricle. The lavage fluid was centrifuged at 1600 x g for 10 min. The supernatant was stored in an ice box, and the level of (TNF)alpha, (IL-6) and TGF-beta1 in the serum was determined by enzyme-linked immunosorbent assay (ELISA). The lavage fluid was stored at -80 degrees C. (3) The level of CD31 and CD34 in lung tissue was detected by flow cytometry. (1) Compared with Sham group, the alveolar hydration of rats in I/R group decreased significantly at the end of the ischemia, 30 min and 60 min of reperfusion (P < 0.05). The airway reactivity increased significantly at the end of the ischemia and 30 min of reperfusion (P < 0.05). Compared with Sham group, the level of
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